Neurofilament H, phosphorylated (NF-H, NFH, NEFH) BioAssay(TM) ELISA Kit

Produktübersicht

• Artikelname: Neurofilament H, phosphorylated (NF-H, NFH, NEFH) BioAssay(TM) ELISA Kit
• Artikelnummer: USB-031402
• Hersteller Artikelnummer: 031402
• Alternativnummer: USB-031402-96
• Hersteller: US Biological
• Kategorie: Kits/Assays

Produktbeschreibung

The Neurofilament H, phosphorylated (NF-H, NFH, NEFH) BioAssay(TM) ELISA Kit measures the levels of phosphorylated NF-H in human, bovine, chicken, equine, mouse and rat serum, plasma and cerebrospinal fluid. Quality control pNF-H is purified from bovine spinal cord. This ELISA assay is intended for research use only. Principle of the Assay: This ELISA Kit provides a convenient method for measuring level of protein targets within a single, small-volume sample. Neurofilament H, phosphorylated (NF-H, NFH, NEFH) BioAssay(TM) ELISA Kit is a 2-site sandwich enzyme linked immunoassay providing a plate pre-coated with mouse monoclonal capture antibody against pNF-H. The end-user incubates the samples on the plate and any pNF-H protein in the sample binds to the capture antibody. The end-user then incubates with a solution containing another mouse monoclonal detection antibody conjugated with HRP, which binds to the captured pNF-H. The end-user adds a developer solution that produces a colored product in wells containing HRP, and the intensity of the color corresponds to the amount of pNF-H in the sample. Finally, the end-user stops the reaction by adding H2SO4. The color changes from blue to yellow and can be quantified by measurement on a suitable ELISA plate reader using an absorbance wave length of 450nm. Kit Components: 031402A: Microtiter Strips, 1x96 wells. Precoated with anti-pNF-H 031402B: Detection Antibody (HRP), 4000X , 1x6ul 031402C: Standard (Bovine), 5ug/ml, 1x10ul 031402D: Blocking Protein, 1x500ug 031402E: TBS-Tween Solution, 10X, 1x5ml 031402F: TMB Substrate,1x12ml Storage and Stability: Store *031402B and *031402C at -20C. Store all other components at 4C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Detection Range: 0.156-10ng/ml Sensitivity: 70pg/ml Precision: Intra-assay CV: <6% Inter-assay CV: <10%