Cell-permeable, non-selective broad-spectrum caspase inhibitor. Binds irreversibly to the catalytic site of caspase proteases. The peptide is O-methylated in the P1 position on aspartic acid, providing enhanced stability and increased cell permeability. Inhibits ICE-family protease/caspase processing, leading to apoptosis and autophagy induction. Decreases proteasome activity. Potent inhibitor of caspase-1 activation in NLRP3-induced cells. Used in apoptosis and inflammasome studies. Synonyms: Z-VAD(OMe)-FMK, Z-Val-Ala-DL-Asp(OMe)-FMK, pan-Caspase Inhibitor CAS No: 187389-52-2 Molecular Weight: 467.5 Sequence: Z-Val-Ala-DL-Asp(OMe)-fluoromethylketone Molecular Formula: C22H30FN3O7 Solubility: DMSO, acetonitrile or dimethyl formamide. Usage: Prepare a 10mM stock solution of the caspase inhibitor in high quality DMSO. Add 2ul of stock solution to 1ml of culture medium containing cells to give 20uM final concentration. Effective final concentrations are estimated to be 5-20uM. If a particular inhibitor is applied at higher concentrations, specificity for an individual caspase or even for the caspase family (>100uM) might be compromised. DMSO concentrations above 0.2% may cause cellular toxicity, thus masking the effect of the caspase inhibitor. For in vivo experiments extending 12 to 48 hours, fresh inhibitor may have to be added (injected) due to inactivation by reaction with cysteine protease. Appearance: Supplied as a white solid. Purity: 95% (HPLC) Storage and Stability: May be stored at 4C for short-term only. Stable for 12 months after receipt when stored at -20C. Keep cool and dry. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molekulargewicht:
467.49
Reinheit:
95% (HPLC)
Formulierung:
Supplied as a white solid.
CAS Nummer:
[187389-52-2]
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