Cas9, Recombinant, Streptococcus pyogenes, His-Tag
Artikelnummer:
USB-045803
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| Artikelname: | Cas9, Recombinant, Streptococcus pyogenes, His-Tag |
| Artikelnummer: | USB-045803 |
| Hersteller Artikelnummer: | 045803 |
| Alternativnummer: | USB-045803-50,USB-045803-100 |
| Hersteller: | US Biological |
| Kategorie: | Molekularbiologie |
| Clustered regularly interspaced short palindromic repeat (CRISPR) and associated CRISPR-associated nuclease (cas) proteins comprise the CRISPR/cas systems that are responsible for adaptive immunity in many bacteria and archaea. Three types of CRISPR/cas systems have been identified to date, which have been designated type I, II and III. Cas9 is an RNA guided DNA nuclease associated with the type II CRISPR/Cas adaptive immune system. In prokaryotes, the CRISPR/Cas9 system provides protection against viruses and plasmids by targeting foreign nucleic acids with short complementary single stranded RNA that localizes the Cas9 nuclease to the target DNA sequence, introducing double strand breaks at specific locations within the genomic DNA. Recombinant protein corresponding to aa2-1368(end) from Streptococcus pyogenes CRISPR associated protein 9 (Cas9), fused to N-terminal HIs-tag, expressed in E. coli. Applications: Suitable for use to form CRISPR RNP complexes for in vitro cleavage assays to identify sgRNAs with the highest cutting efficiencies. Other applications not tested. Assay Conditions: The Cas9 nuclease was incubated with sgRNA at 37C for 10 minutes to form a functional RNP complex. A purified PCR product was then added at 37C for 60 minutes, before Cas9 activity was visualized by agarose gel. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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