DNA Polymerase, gamma, Recombinant, Human, His-FLAG-Tag, aa2-1239 (POLG)

Produktübersicht

• Artikelname: DNA Polymerase, gamma, Recombinant, Human, His-FLAG-Tag, aa2-1239 (POLG)
• Artikelnummer: USB-045804
• Hersteller Artikelnummer: 045804
• Alternativnummer: USB-045804-100
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

Polymerases are the enzymes responsible for synthesizing nucleic acids. Polymerase gamma, also known as DNA polymerase subunit gamma, POLG or POLG1, belongs to the Family A of DNA polymerases and corresponds to the catalytic subunit of the mitochondrial DNA polymerase. It works in conjunction with POLG2 to replicate mitochondrial DNA. In addition to its DNA polymerase activity, it has a 3-5 exonuclease and a 5dRP lyase activity for proofreading and repair. Mutations in polymerase gamma cause mitochondrial dysfunction, as observed in SANDO (sensory ataxia neuropathy dysarthia and ophthalmoparesis), AHS (Alpers-Huttenlocher Syndrome) and MNGIE (mitochondrial neurogastrointestinal encephalopathy syndrome). Alternatively, the inhibition of DNA synthesis can be used as a therapeutic approach for diseases in which cell division or energetic needs are uncontrolled, such as cancer. Further studies into DNA polymerase inhibitors will bring new therapy options to cancer patients. Recombinant human DNA polymerase gamma (gamma), full length encompassing amino acids 2-1239 (end)fused to N-terminal 6X His-Tag followed by a FLAG-tag, expressed in Sf9 cell expression system. Molecular Weight: ~141kD Assay Conditions: Assay was performed using Human DNA Polymerase Assay Kit and with various amounts of DNA Polymerase gamma enzyme. The assay was run in 384-well format with a reaction volume of 15ul supplemented with 15ul of detection reagent (total 30ul) prior plate reading. Plate was incubated for 1 hour at 37C prior detection. Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap

Produkteigenschaften

• Molekulargewicht: 141
• NCBI: 002693
• UniProt: P54098
• Reinheit: 90%
• Formulierung: Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM KCl, 100ug/ml FLAG peptide, 0.04% Tween 20, 20% glycerol.