Selenoprotein W, Recombinant, Human, His-Tag, aa1-87

Produktübersicht

• Artikelname: Selenoprotein W, Recombinant, Human, His-Tag, aa1-87
• Artikelnummer: USB-045980
• Hersteller Artikelnummer: 045980
• Alternativnummer: USB-045980-20,USB-045980-100
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

SEPW1 is a selenoprotein, which contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the uGA codon that normally signals translation termination. The 3 uTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of uGA as a Sec codon rather than as a stop signal. This protein shows highest expression in skeletal muscle and heart, and may be involved in oxidation-reduction reactions. A retroprocessed pseudogene, SEPW1P, has been identified and mapped to chromosome 1p35-34. Recombinant human SEPW1 protein, fused to His-Tag at N -terminal, was expressed in E. coli and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa1-87 from human Selenoprotein W, fused to His-Tag at N-terminal expressed in E.coli. Molecular Weight: ~11.8kD (110aa) Amino Acid Sequence: MALAVRV VYCGACGYKS KYLQLKKKLE DEFPGRLDIC GEGTPQATGF FEVMVAGKLI HSKKKGDGYV DTESKFLKLV AAIKAALAQG Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Molekulargewicht: 11.8
• NCBI: 003000
• UniProt: P63302
• Reinheit: 95% (SDS-PAGE)
• Formulierung: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.15 Sodium Chloride, 1mM DTT, 20% glycerol.