IL-8, Recombinant, Canine, His-Tag, aa28-101

Produktübersicht

• Artikelname: IL-8, Recombinant, Canine, His-Tag, aa28-101
• Artikelnummer: USB-045990
• Hersteller Artikelnummer: 045990
• Alternativnummer: USB-045990-20,USB-045990-100
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

IL-8/CXCL8, also known as Interleukin-8, GCP-1, and NAP-1 is a member of the CXC family of chemokines family which is widely expressed proinflammatory. IL-8 is a potent neutrophil chemotactic factor. It attracts neutrophils, basophils, and T-cells, but not monocytes. This protein has two primary functions. It induces chemotaxis in target cells, primarily neutrophils but also other granulocytes, causing them to migrate toward the site of infection. IL-8 also stimulates phagocytosis once they have arrived. IL-8 is also known to be a potent promoter of angiogenesis. In target cells, IL-8 induces a series of physiological responses required for migration and phagocytosis, such as increases in intracellular Ca2+, exocytosis, and the respiratory burst. Among the eceptors on the surface membrane capable of binding IL-8, the most frequently studied receptors are CXCR1 and CXCR2. Expression and affinity for IL-8 differs between the two receptors (CXCR1 > CXCR2). Recombinant canine IL 8/CXCL8, fused to His-Tag at C-terminal, was expressed in HEK293 cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa28-101 from canine IL-8, fused to His-Tag at C-terminal, expressed in HEK293. Molecular Weight: ~9.4kD (80aa) Amino Acid Sequence: VSSELRCQCI KTHSTPFHPK YIKELRVIDS GPHCENSEII VKLFNGNEVC LDPKEKWVQK VVQIFLKKAE KQDP Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Molekulargewicht: 9.4
• NCBI: 001003200
• UniProt: P41324
• Reinheit: 90% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
• Formulierung: Supplied as a liquid in PBS, pH 7.4, 20% glycerol.