IL-21, Recombinant, Mouse, aa18-146
Artikelnummer:
USB-045996
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| Artikelname: | IL-21, Recombinant, Mouse, aa18-146 |
| Artikelnummer: | USB-045996 |
| Hersteller Artikelnummer: | 045996 |
| Alternativnummer: | USB-045996-20,USB-045996-100 |
| Hersteller: | US Biological |
| Kategorie: | Molekularbiologie |
| IL-21, also known as Interleukin-21, is produced from activated helper T cells such as Th17 cells and is a member of the common-gamma chain family of cytokines that includes IL-2, IL-4, IL-7, IL-9, and IL-15. Previous studies have shown that IL-21 plays an important role in the regulation of both innate and adaptive immune responses. IL-21 is critical for NK cell activation and expansion. IL-21 also promotes terminal B cell differentiation into plasma cells and antibody production. Furthermore, IL-21 enhances antigen-specific CD8 T cell expansion and the differentiation of follicular helper T cells in vivo. IL-21 also promotes the differentiation of the inflammatory Th17 lineage and contributes to autoimmunity. IL-21 isoform is an alternative splice variant form of IL-21 that we previously identified and reported to have a similar function as original IL-21. Follicular helper T cells also produce IL-21 to activate and differentiate B cells. Recombinant mouse IL-21, was expressed in E. coli and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa18-146 from mouse IL-21, expressed in E.coli. Molecular Weight: ~15kD (130aa) Amino Acid Sequence: MHKSSPQGPD RLLIRLRHLI DIVEQLKIYE NDLDPELLSA PQDVKGHCEH AAFACFQKAK LKPSNPGNNK TFIIDLVAQL RRRLPARRGG KKQKHIAKCP SCDSYEKRTP KEFLERLKWL LQKMIHQHLS Biological Activity: The activity is determined by the IFN-g ELISA in a using NK-92 human natural killer cells. The ED50 range 100ng/ml. Applications: Suitable for use in SDS-PAGE, Bioactivity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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