Ribonuclease A, Recombinant, Human, His-Tag, aa29-156

Produktübersicht

• Artikelname: Ribonuclease A, Recombinant, Human, His-Tag, aa29-156
• Artikelnummer: USB-045998
• Hersteller Artikelnummer: 045998
• Alternativnummer: USB-045998-20,USB-045998-100
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

RNASE1, also known as ribonuclease A, is a member of pancreatic ribonuclease enzyme family. It is a relatively small protein and is a basic protein (pI = 9.63). Also, It has four disulfide bonds in its native state. It cleaves specifically after pyrimidine nucleotides. Cleavage takes place in two steps: first, the 3,5-phosphodiester bond is cleaved to generate a 2,3-cyclic phosphodiester intermediate, second, the cyclic phosphodiester is hydrolyzed to a 3-monophosphate. (For example pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG) The highest activity is exhibited with single stranded RNA. It can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts. Recombinant human RNASE1, fused to His-Tag at C-terminal, was expressed in HEK293 cell and purified by using conventional chromatography techniques. Recombinant protein corresponding to aa29-156 from human Ribonuclease A, fused to His-Tag at C-terminal, expressed in HEK293 cells. Accession Number: NP_937878.1 Molecular Weight: ~15.3kD (134aa) Amino Acid Sequence: KESRAKKFQR QHMDSDSSPS SSSTYCNQMM RRRNMTQGRC KPVNTFVHEP LVDVQNVCFQ EKVTCKNGQG NCYKSNSSMH ITDCRLTNGS RYPNCAYRTS PKERHIIVAC EGSPYVPVHF DASVEDST Specifc Activity: <5x10e4 unit/mg, Unit Definition: Defined as the amount of enzyme that cleaves 1pmole of RNase probe per minute at 25C. Applications: Suitable for use in SDS-PAGE, Enzyme Activity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Molekulargewicht: 15.3
• NCBI: 937878
• UniProt: P07998
• Reinheit: 90% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
• Formulierung: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.