Osteopontin, Recombinant, Mouse, His-Tag, aa17-294

Produktübersicht

• Artikelname: Osteopontin, Recombinant, Mouse, His-Tag, aa17-294
• Artikelnummer: USB-045999
• Hersteller Artikelnummer: 045999
• Alternativnummer: USB-045999-20,USB-045999-100,USB-045999-250
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

Osteopontin, also known as SPP1, is major non-collagenous bone protein that binds tightly to hydroxyapatite. This protein appears to form an integral part of the mineralized matrix. It inhibits bone mineralization and kidney stone formation, and promotes inflammation and cell adhesion and migration. Osteopontin acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin 10. Also it is essential in the pathway that leads to type 1 immunity. It is expressed and secreted by various cells, and has a role in cell adhesion, chemotaxis, prevention of apoptosis, invasion, migration and anchorage independent growth of tumor cells. Recombinant mouse Osteopontin, fused to His-Tag at C-terminal, was expressed in HEK293 cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa17-294 from mouse Osteopontin, fused to His-Tag at C-terminal, expressed in HEK293. Molecular Weight: ~31.8kD (287aa) Amino Acid Sequence: LPVKVTD SGSSEEKLYS LHPDPIATWL VPDPSQKQNL LAPQNAVSSE EKDDFKQETL PSNSNESHDH MDDDDDDDDD DGDHAESEDS VDSDESDESH HSDESDETVT ASTQADTFTP IVPTVDVPNG RGDSLAYGLR SKSRSFQVSD EQYPDATDED LTSHMKSGES KESLDVIPVA QLLSMPSDQD NNGKGSHESS QLDEPSLETH RLEHSKESQE SADQSDVIDS QASSKASLEH QSHKFHSHKD KLVLDPKSKE DDRYLKFRIS HELESSSSEV N Biological Activity: Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. When cells are added to mouse SPP1 coated plates. The ED50 range 1.5ug/ml. Applications: Suitable for use in SDS-PAGE, Bioactivity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Molekulargewicht: 31.8
• NCBI: 033289
• UniProt: P10923
• Reinheit: 90% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
• Formulierung: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.