Acetylcholinesterase, Recombinant, Human, His-Tag, aa32-614

Produktübersicht

• Artikelname: Acetylcholinesterase, Recombinant, Human, His-Tag, aa32-614
• Artikelnummer: USB-046005
• Hersteller Artikelnummer: 046005
• Alternativnummer: USB-046005-20,USB-046005-100
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

ACHE, also known as Acetylcholinesterase, is a member of the type-B carboxylesterase/lipase family. It is an enzyme that catalyzes the breakdown of acetylcholine and of some other choline esters that function as neurotransmitters. During neurotransmission, ACH is released from the presynaptic neuron into the synaptic cleft and binds to ACH receptors on the post-synaptic membrane, relaying the signal from the nerve. ACHE, also located on the post-synaptic membrane, terminates the signal transmission by hydrolyzing ACH. This protein is thought to be involved in the pathology of Alzheimers disease (AD) by accelerating the assembly of A beta peptides into fibrillar species through forming complexes with A beta via the peripheral anionic site on ACHE. ACHE inhibitors have been used to delay symptoms of AD patients by virtue of their ability to enhance ACH availability, as well as reduce amyloidogenesis and subsequent neurotoxicity. Recombinant human ACHE, fused to His-Tag at C-terminal, was expressed in HEK293 cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa32-614 from human Acetylcholinesterase, fused to His-Tag at C-terminal, expressed in HEK293. Molecular Weight: ~65.6kD (592aa) Amino Acid Sequence: EGREDAE LLVTVRGGRL RGIRLKTPGG PVSAFLGIPF AEPPMGPRRF LPPEPKQPWS GVVDATTFQS VCYQYVDTLY PGFEGTEMWN PNRELSEDCL YLNVWTPYPR PTSPTPVLVW IYGGGFYSGA SSLDVYDGRF LVQAERTVLV SMNYRVGAFG FLALPGSREA PGNVGLLDQR LALQWVQENV AAFGGDPTSV TLFGESAGAA SVGMHLLSPP SRGLFHRAVL QSGAPNGPWA TVGMGEARRR ATQLAHLVGC PPGGTGGNDT ELVACLRTRP AQVLVNHEWH VLPQESVFRF SFVPVVDGDF LSDTPEALIN AGDFHGLQVL VGVVKDEGSY FLVYGAPGFS KDNESLISRA EFLAGVRVGV PQVSDLAAEA VVLHYTDWLH PEDPARLREA LSDVVGDHNV VCPVAQLAGR LAAQGARVYA YVFEHRASTL SWPLWMGVPH GYEIEFIFGI PLDPSRNYTA EEKIFAQRLM RYWANFARTG DPNEPRDPKA PQWPPYTAGA QQYVSLDLRP LEVRRGLRAQ ACAFWNRFLP KLLSATDTLD EAERQWKAEF HRWSSYMVHW KNQFDHYSKQ DRCSDL Biological Activity: Specific activity is > 6,000nmol/min/mg, and is defined as the amount of enzyme that cleaves 1.0nmol acetylthiocholine per minute at pH7.5 at 25C. Applications: Suitable for use in SDS-PAGE, Enzyme Activity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Molekulargewicht: 65.6
• NCBI: 000656
• UniProt: P22303
• Reinheit: 95% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
• Formulierung: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.