TNFRSF7, Recombinant, Human, His-Tag, aa20-191

Produktübersicht

• Artikelname: TNFRSF7, Recombinant, Human, His-Tag, aa20-191
• Artikelnummer: USB-046006
• Hersteller Artikelnummer: 046006
• Alternativnummer: USB-046006-20,USB-046006-50,USB-046006-100,USB-046006-250
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

CD27, as known as TNFRSF7, is a member of the tumor necrosis factor receptor superfamily. It has been shown to play roles in lymphoid proliferation, differentiation, and apoptosis.It has an important role in the generation of T cell immunity and is an robust marker for normal memory B cells. Also, this protein plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis. As a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD 70 on lymphocytes and dendritic cells. It binds to ligand CD70, and CD27/CD70 co-stimulatory system promote T, B and NK cell responses and particularly the CTL response, and is a target in cancer immunotherapy. Recombinant human CD27, fused to hIgG-His-Tag at C-terminal, was expressed in HEK293 and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa20-191 from human TNFRSF7, fused to hlgG-His-Tag at C-terminal, expressed in HEK293. Molecular Weight: ~46.2kD (411aa) Amino Acid Sequence: ATPAPKSCPE RHYWAQGKLC CQMCEPGTFL VKDCDQHRKA AQCDPCIPGV SFSPDHHTRP HCESCRHCNS GLLVRNCTIT ANAECACRNG WQCRDKECTE CDPLPNPSLT ARSSQALSPH PQPTHLPYVS EMLEARTAGH MQTLADFRQL PARTLSTHWP PQRSLCSSDF IR Biological Activity: Measured by its binding ability in a functional ELISA with Human CD27 Ligand/TNFSF7. The ED50 range 0.8ug/ml. Applications: Suitable for use in SDS-PAGE, Bioactivity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Molekulargewicht: 46.2
• NCBI: 001233
• UniProt: P26842
• Reinheit: 95% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
• Formulierung: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.