Akt3, Recombinant, Human, His-Tag, aa1-479

Produktübersicht

• Artikelname: Akt3, Recombinant, Human, His-Tag, aa1-479
• Artikelnummer: USB-046007
• Hersteller Artikelnummer: 046007
• Alternativnummer: USB-046007-20,USB-046007-100
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

Akt3, also known as RAC-gamma serine/threonine-protein kinase, is a member of the Akt subfamily of serine/threonine protein kinases. It is one of 3 closely related serine/threonine-protein kinases (Akt1, Akt2 and Akt3) called the Akt kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. Also this is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Akt3 is the least studied Akt isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. Recombinant human Akt3, fused to His-Tag at C -terminal, was expressed in HEK293 and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa1-479 from human Akt3, fused to His-Tag at C-terminal, expressed in HEK293. Molecular Weight: ~56.5kD (485aa) Amino Acid Sequence: MSDVTIVKEG WVQKRGEYIK NWRPRYFLLK TDGSFIGYKE KPQDVDLPYP LNNFSVAKCQ LMKTERPKPN TFIIRCLQWT TVIERTFHVD TPEEREEWTE AIQAVADRLQ RQEEERMNCS PTSQIDNIGE EEMDASTTHH KRKTMNDFDY LKLLGKGTFG KVILVREKAS GKYYAMKILK KEVIIAKDEV AHTLTESRVL KNTRHPFLTS LKYSFQTKDR LCFVMEYVNG GELFFHLSRE RVFSEDRTRF YGAEIVSALD YLHSGKIVYR DLKLENLMLD KDGHIKITDF GLCKEGITDA ATMKTFCGTP EYLAPEVLED NDYGRAVDWW GLGVVMYEMM CGRLPFYNQD HEKLFELILM EDIKFPRTLS SDAKSLLSGL LIKDPNKRLG GGPDDAKEIM RHSFFSGVNW QDVYDKKLVP PFKPQVTSET DTRYFDEEFT AQTITITPPE KYDEDGMDCM DNERRPHFPQ FSYSASGRE Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Molekulargewicht: 56.5
• NCBI: 005456
• UniProt: Q9Y243
• Reinheit: 90% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
• Formulierung: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.