CCL2, Recombinant, Human, His-Tag, aa24-99

Produktübersicht

• Artikelname: CCL2, Recombinant, Human, His-Tag, aa24-99
• Artikelnummer: USB-046013
• Hersteller Artikelnummer: 046013
• Alternativnummer: USB-046013-20,USB-046013-100
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

CCL2(Chemokine ligand 2), as known as MCP-1(monocyte chemotactic protein-1), is a small cytokine belonging to the CC chemokine family. This protein recruits monocytes, memory T cells, and dendritic cells to sites of tissue injury and infection. It binds to chemokine receptors CCR2 and CCR4. It also induces enzyme and cytokine release by monocytes, NK cells and lymphocytes, and histamine release by basophils, primarily due to interaction with CCR2 receptors on these cells. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. Recombinant human CCL2/JE/MCP-1, fused to His-Tag at C-terminal, was expressed in HEK293 and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa24-99 from human CCL2, fused to His-Tag at C-terminal, expressed in HEK293. Molecular Weight: ~9.5kD (82aa) Amino Acid Sequence: QPDAINAPVT CCYNFTNRKI SVQRLASYRR ITSSKCPKEA VIFKTIVAKE ICADPKQKWV QDSMDHLDKQ TQTPKT Biological Activity: Measured its ability to chemoattract using THP-1 human acute monocytic leukemia cells. The ED50 range 30ng/ml. Applications: Suitable for use in SDS-PAGE, Bioactivity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Molekulargewicht: 9.5
• NCBI: 002973
• UniProt: P13500
• Reinheit: 90% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
• Formulierung: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.