TrkA, Recombinant, Rat, His-Tag, aa35-418

Produktübersicht

• Artikelname: TrkA, Recombinant, Rat, His-Tag, aa35-418
• Artikelnummer: USB-046030
• Hersteller Artikelnummer: 046030
• Alternativnummer: USB-046030-20,USB-046030-100
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

TrkA, also known as Tyrosine kinase receptor A, is a member of the neurotrophic tyrosine kinase receptor(NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. It leads to cell differentiation and may play a role in specifying sensory neuron subtypes. It has a crucial role in the development and function of the nociceptive reception system as well as the establishment of thermal regulation via sweating. In one study conducted on two rat models, an inhibition of TrkA with AR786 led to a reduction in joint swelling, joint damage, and pain caused by inflammatory arthritis. Recombinant rat TrkA, fused to hIgG-His-Tag at C-terminal, was expressed in HEK293 cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa35-418 from rat TrkA, fused to hlgG-His-Tag at C-terminal, expressed in HEK293. Molecular Weight: ~69kD (623aa) Amino Acid Sequence: SCRETCCPVG PSGLRCTRAG TLNTLRGLRG AGNLTELYVE NQRDLQRLEF EDLQGLGELR SLTIVKSGLR FVAPDAFHFT PRLSHLNLSS NALESLSWKT VQGLSLQDLT LSGNPLHCSC ALLWLQRWEQ EDLCGVYTQK LQGSGSGDQF LPLGHNNSCG VPSVKIQMPN DSVEVGDDVF LQCQVEGQAL QQADWILTEL EGTATMKKSG DLPSLGLTLV NVTSDLNKKN VTCWAENDVG RAEVSVQVSV SFPASVHLGK AVEQHHWCIP FSVDGQPAPS LRWFFNGSVL NETSFIFTQF LESALTNETM RHGCLRLNQP THVNNGNYTL LAANPYGQAA ASIMAAFMDN PFEFNPEDPI PVSFSPVDTN STSRDPVEKK DETP Biological Activity: Measured by ability to inhibit NGF-induced proliferation assay using TF-1 human erythroleukemic cells in the presence of 0.5ng/ml of rat NGF. The ED50 range 5ng/ml. Applications: Suitable for use in SDS-PAGE, Bioactivity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Molekulargewicht: 69
• NCBI: 067600
• UniProt: P35739
• Reinheit: 90% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
• Formulierung: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.