Cathepsin D, Recombinant, Human, His-Tag, aa21-412

Produktübersicht

• Artikelname: Cathepsin D, Recombinant, Human, His-Tag, aa21-412
• Artikelnummer: USB-046031
• Hersteller Artikelnummer: 046031
• Alternativnummer: USB-046031-20,USB-046031-50,USB-046031-100,USB-046031-250
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

Cathepsin D, also known as lysosomal aspartyl protease, is a member of the peptidase C1 family, which is a normal and major component of lysosomes, and is found in almost all cells and tissues of mammals. The main physiological functions of cathepsin D consist of metabolic degradation of intracellular proteins, activation and degradation of polypeptide hormones and growth factors, activation of enzymatic precursors, processing of enzyme activators and inhibitors, brain antigen processing and regulation of programmed cell death. In addition, it secreted from human prostate carcinoma cells are responsible for the generation of angiostatin, a potent endogenous inhibitor of angiogenesis, suggesting its contribution to the prevention of tumor growth and angiogenesis-dependent growth of metastases. Recombinant human Cathepsin D, fused to His-Tag at C- terminal, was expressed in HEK293 cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa21-412 from human Cathepsin D, fused to His-Tag at C-terminal, expressed in HEK293. Molecular Weight: ~43.4kD (398aa) Amino Acid Sequence: LVRIPLHKFT SIRRTMSEVG GSVEDLIAKG PVSKYSQAVP AVTEGPIPEV LKNYMDAQYY GEIGIGTPPQ CFTVVFDTGS SNLWVPSIHC KLLDIACWIH HKYNSDKSST YVKNGTSFDI HYGSGSLSGY LSQDTVSVPC QSASSASALG GVKVERQVFG EATKQPGITF IAAKFDGILG MAYPRISVNN VLPVFDNLMQ QKLVDQNIFS FYLSRDPDAQ PGGELMLGGT DSKYYKGSLS YLNVTRKAYW QVHLDQVEVA SGLTLCKEGC EAIVDTGTSL MVGPVDEVRE LQKAIGAVPL IQGEYMIPCE KVSTLPAITL KLGGKGYKLS PEDYTLKVSQ AGKTLCLSGF MGMDIPPPSG PLWILGDVFI GRYYTVFDRD NNRVGFAEAA RL Biological Activity: Specific activity is > 20pmol/min/ug, and is defined as the amount of enzyme that cleaves 1pmol of Mca-PLGL-Dpa-AR-NH2 per minute at pH 3.5 at 25C. Applications: Suitable for use in SDS-PAGE, Enzyme Activity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Molekulargewicht: 43.4
• NCBI: 001900
• UniProt: P07339
• Reinheit: 95% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
• Formulierung: Supplied as a liquid in 50mM MES, pH 5.5, 100mM sodium chloride, 20% glycerol.