MMP-9, Recombinant, Rat, His-Tag, aa20-708

Produktübersicht

• Artikelname: MMP-9, Recombinant, Rat, His-Tag, aa20-708
• Artikelnummer: USB-046051
• Hersteller Artikelnummer: 046051
• Alternativnummer: USB-046051-20,USB-046051-50,USB-046051-100,USB-046051-250
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

MMP-9, also known matrix metalloproteinase-9, is one of the matrix metalloproteinases superfamily which is zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. It degrades many substrates such as gelatin, collagens, elastin and proteoglycan core protein which appears to be involved in invasive ability. This protein also plays an essential role in leukocyte migration and in bone osteoclastic resorption. It plays an important role in angiogenesis and neovascularization and so appears to be involved in the remodeling associated with malignant glioma neovascularization. Recombinant rat MMP-9 protein, fused to His-Tag at C-terminal, was expressed in HEK293 cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa20-708 from rat MMP-9, fused to His-Tag at C-terminal, expressed in HEK293. Molecular Weight: ~77.2kD (695aa) Amino Acid Sequence: APHQRQPTYV VFPRDLKTSN LTDTQLAEDY LYRYGYTRAA QMMGEKQSLR PALLMLQKQL SLPQTGELDS ETLKAIRSPR CGVPDVGKFQ TFEGDLKWHH HNITYWIQSY TEDLPRDVID DSFARAFAVW SAVTPLTFTR VYGLEADIVI QFGVAEHGDG YPFDGKDGLL AHAFPPGPGI QGDAHFDDDE LWSLGKGAVV PTYFGNANGA PCHFPFTFEG RSYLSCTTDG RNDGKPWCGT TADYDTDRKY GFCPSENLYT EHGNGDGKPC VFPFIFEGHS YSACTTKGRS DGYRWCATTA NYDQDKLYGF CPTRADVTVT GGNSAGEMCV FPFVFLGKQY STCTGEGRSD GRLWCATTSN FDADKKWGFC PDQGYSLFLV AAHEFGHALG LDHSSVPEAL MYPMYHYHED SPLHEDDIKG IQHLYGRGSK PDPRPPATTA AEPQPTAPPT MCPTAPPMAY PTGGPTVAPT GAPSPGPTGP PTAGPSEAPT ESSTPVDNPC NVDVFDAIAD IQGALHFFKD GRYWKFSNHG GSQLQGPFLI ARTWPALPAK LNSAFEDPQS KKIFFFSGRK MWVYTGQTVL GPRSLDKLGL GSEVTLVTGL LPRRGGKALL ISRERIWKFD LKSQKVDPQS VTRLDNEFSG VPWNSHNVFH YQDKAYFCHD KYFWRVSFHN RVNQVDHVAY VTYDLLQCP Biological Activity: Specific activity is > 2000pmol/min/ug, and is defined as the amount of enzyme that cleaves 1pmol of Mca-PLGL-Dpa-AR-NH2 per minute at pH 7.5 at 25C. Applications: Suitable for use in SDS-PAGE, Enzyme Activity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Molekulargewicht: 77.2
• NCBI: 112317
• UniProt: P50282
• Reinheit: 90% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
• Formulierung: Supplied as a liquid in 20mM Tris-HCl , pH 7.5, 1mM calcium chloride, 100mM sodium chloride, 10% glycerol.