Insulysin, Recombinant, Human, His-Tag, aa42-1019

Produktübersicht

• Artikelname: Insulysin, Recombinant, Human, His-Tag, aa42-1019
• Artikelnummer: USB-046069
• Hersteller Artikelnummer: 046069
• Alternativnummer: USB-046069-20,USB-046069-50,USB-046069-100,USB-046069-250
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

Insulysin/IDE, also known insulin-degrading enzyme, is a zinc metallopeptidase of the inverzincin family. This enzyme catalyses the degradation reaction of insulin, glucagon and other polypeptides. It is present in mammals and in many arthropods such as the fly Drosophila melanogaster. It is also expressed in many tissues, with the highest levels in liver, kidney, brain, and testis. It has been shown to degrade the amyloid beta peptide, which polymerizes into the plaques associated with Alzheimers disease. Therefore, the deficiency of IDE activity may contribute to the pathogenesis of type 2 diabetes mellitus and Alzheimers disease. Recombinant human Insulysin/IDE, fused to His-Tag at C-terminal, was expressed in insect cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa42-1019 from human Insulysin, fused to His-Tag at C-terminal, expressed in Baculovirus. Molecular Weight: ~114kD (984aa) Amino Acid Sequence: MNNPAIKRIG NHITKSPEDK REYRGLELAN GIKVLLISDP TTDKSSAALD VHIGSLSDPP NIAGLSHFCE HMLFLGTKKY PKENEYSQFL SEHAGSSNAF TSGEHTNYYF DVSHEHLEGA LDRFAQFFLC PLFDESCKDR EVNAVDSEHE KNVMNDAWRL FQLEKATGNP KHPFSKFGTG NKYTLETRPN QEGIDVRQEL LKFHSAYYSS NLMAVCVLGR ESLDDLTNLV VKLFSEVENK NVPLPEFPEH PFQEEHLKQL YKIVPIKDIR NLYVTFPIPD LQKYYKSNPG HYLGHLIGHE GPGSLLSELK SKGWVNTLVG GQKEGARGFM FFIINVDLTE EGLLHVEDII LHMFQYIQKL RAEGPQEWVF QECKDLNAVA FRFKDKERPR GYTSKIAGIL HYYPLEEVLT AEYLLEEFRP DLIEMVLDKL RPENVRVAIV SKSFEGKTDR TEEWYGTQYK QEAIPDEVIK KWQNADLNGK FKLPTKNEFI PTNFEILPLE KEATPYPALI KDTAMSKLWF KQDDKFFLPK ACLNFEFFSP FAYVDPLHCN MAYLYLELLK DSLNEYAYAA ELAGLSYDLQ NTIYGMYLSV KGYNDKQPIL LKKIIEKMAT FEIDEKRFEI IKEAYMRSLN NFRAEQPHQH AMYYLRLLMT EVAWTKDELK EALDDVTLPR LKAFIPQLLS RLHIEALLHG NITKQAALGI MQMVEDTLIE HAHTKPLLPS QLVRYREVQL PDRGWFVYQQ RNEVHNNCGI EIYYQTDMQS TSENMFLELF CQIISEPCFN TLRTKEQLGY IVFSGPRRAN GIQGLRFIIQ SEKPPHYLES RVEAFLITME KSIEDMTEEA FQKHIQALAI RRLDKPKKLS AECAKYWGEI ISQQYNFDRD NTEVAYLKTL TKEDIIKFYK EMLAVDAPRR HKVSVHVLAR EMDSCPVVGE FPCQNDINLS QAPALPQPEV IQNMTEFKRG LPLFPLVKPH INFMAAKL Biological Activity: Specific activity is > 3000pmol/min/ug in which one unit will convert 1.0 pmole of Mca-RPPGFSAFK(Dnp)-OH to MCA-Pro-Leu-OH per minute at pH 7.5 at 25C. Applications: Suitable for use in SDS-PAGE, Enzyme Activity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Molekulargewicht: 114
• NCBI: 004960
• UniProt: P14735
• Reinheit: 90% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
• Formulierung: Supplied as a liquid in 20mM Tris-HCl, pH 7.5 100mM sodium chloride, 0.05% Brij35, 10% glycerol.