Sialoprotein II, Recombinant, Human, His-Tag, aa17-317

Produktübersicht

• Artikelname: Sialoprotein II, Recombinant, Human, His-Tag, aa17-317
• Artikelnummer: USB-046070
• Hersteller Artikelnummer: 046070
• Alternativnummer: USB-046070-20,USB-046070-100
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

IBSP, also known as Sialoprotein II, is a monomeric noncollagenous member of the SIBLING family of extracellular matrix (ECM) proteins. It is synthesized by platelets, osteoblasts, osteocytes, odontoblasts, osteoclasts and bone marrow stromal cells. It is a key mediator of the hypertrophic chondrocytes-induced angiogenesis. It was associated with the severity of histological cartilage lesions and with vascular density at the osteochondral junction. Recombinant human IBSP/Sialoprotein II, fused to His-Tag at C-terminal, was expressed in HEK293 cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa17-317 from human Sialoprotein II, fused His-Tag at C-terminal, expressed in HEK293. Molecular Weight: ~34.3kDa (307aa) Amino Acid Sequence: FSMKNLHRRV KIEDSEENGV FKYRPRYYLY KHAYFYPHLK RFPVQGSSDS SEENGDDSSE EEEEEEETSN EGENNEESNE DEDSEAENTT LSATTLGYGE DATPGTGYTG LAAIQLPKKA GDITNKATKE KESDEEEEEE EEGNENEESE AEVDENEQGI NGTSTNSTEA ENGNGSSGGD NGEEGEEESV TGANAEDTTE TGRQGKGTSK TTTSPNGGFE PTTPPQVYRT TSPPFGKTTT VEYEGEYEYT GANEYDNGYE IYESENGEPR GDNYRAYEDE YSYFKGQGYD GYDGQNYYHH Q Biological Activity: Measured by the ability of the immobilized protein to support the adhesion of MCF7 human breast cancer cells. When cells are added to Human IBSP coated plates 3ug/ml. This effect is more to 40%. Applications: Suitable for use in SDS-PAGE, Bioactivity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Molekulargewicht: 34.3
• NCBI: 004958
• UniProt: P21815
• Reinheit: 90% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
• Formulierung: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.