Trypsin 3, Recombinant, Human, His-Tag, aa16-247

Produktübersicht

• Artikelname: Trypsin 3, Recombinant, Human, His-Tag, aa16-247
• Artikelnummer: USB-046071
• Hersteller Artikelnummer: 046071
• Alternativnummer: USB-046071-20,USB-046071-100
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

PRSS3, also known as trypsin-3, is a member of the trypsin family of serine proteases. It is specialized for the degradation of trypsin inhibitors and may be involved in defensin processing, including DEFA5. This protein is expressed in the brain and pancreas and is resistant to common trypsin inhibitors. It is active on peptide linkages involving the carboxyl group of lysine or arginine. Compared to PRSS1 and 2, one intriguing feature of PRSS3 is its resistance to polypeptide trypsin inhibitors, such as the Kunitz-type soybean trypsin inhibitor or the Kazal-type pancreatic secretory trypsin inhibitor. It has been proposed to be degradation of trypsin inhibitors, which facilitates the digestion of those foods rich in these proteins. Recombinant human Trypsin 3/PRSS3, fused to His-Tag at C-terminal, was expressed in HEK293 cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa16-247 from human Trypsin 3, fused to His-Tag at C-terminal, expressed in HEK293. Molecular Weight: ~26kD (238aa) Amino Acid Sequence: VPFDDDDKIV GGYTCEENSL PYQVSLNSGS HFCGGSLISE QWVVSAAHCY KTRIQVRLGE HNIKVLEGNE QFINAAKIIR HPKYNRDTLD NDIMLIKLSS PAVINARVST ISLPTAPPAA GTECLISGWG NTLSFGADYP DELKCLDAPV LTQAECKASY PGKITNSMFC VGFLEGGKDS CQRDSGGPVV CNGQLQGVVS WGHGCAWKNR PGVYTKVYNY VDWIKDTIAA NS Biological Activity: Specific activity is > 10,000pmol/min/ug, and is defined as the amount of enzyme that cleaves 1pmol of Mca-RPKPVE-Nval-WRK(Dnp)-NH2 per minute at pH 8.0 at 37°C. Applications: Suitable for use in SDS-PAGE, Enzyme Activity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Molekulargewicht: 26
• NCBI: 002762
• UniProt: P35030
• Reinheit: 95% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
• Formulierung: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.