Synthetic peptide corresponding to amino acid residues in the N-terminal region of Aspergillus Terreus Blasticidin S Deaminase, conjugated to KLH.
Retroviral vectors are commonly used for gene expression in vitro and in vivo. The structure of these vectors usually consists of one gene of interest and a selectable marker gene. Fast selection without damaging cells is a critical step for significant gene expression. Blasticidin S deaminase (Bsd) isolated from Bacillus cereus has a neutralizing action on the highly toxic antibiotic blasticidin. A variety of commercially available retroviral vectors, such lentiviral expression systems, utilize Bsd expression for selection using blasticidin. This selectable marker allows selection of stable mammalian cell lines in as little as one week. 046230 is a Bsd antibody that can detect Bsd expression in cells transfected with blasticidin-selectable constructs. This antibody is useful for detection of successful gene transfer in constructs that utilize blasticidin selection. Applications: Suitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:2000 Western Blot: 1:1000 Immunocytochemistry: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
