Recombinant protein corresponding to His117~Gly232 from human Uroplakin 1A, with a N-terminal His-Tag, expressed in E. coli. Molecular Weight: ~17.0kD Applications: Suitable for use as a positive control, immunogen, SDS-PAGE and Western Blot. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied as a lyophilized powder in 100mMNaHCO3, 500mM sodium chloride, pH 8.3, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300. Reconstitute in dH2O to a concentration of 0.1-1mg/ml. Do not vortex.
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