Choline Kinase Beta (CHKb), Recombinant, Mouse, His-Tag

Artikelnummer: USB-050278
Artikelname: Choline Kinase Beta (CHKb), Recombinant, Mouse, His-Tag
Artikelnummer: USB-050278
Hersteller Artikelnummer: 050278
Alternativnummer: USB-050278-10,USB-050278-50,USB-050278-100,USB-050278-200
Hersteller: US Biological
Kategorie: Molekularbiologie
Applikation: WB
Recombinant protein corresponding to Phe238~Ser394 from mouse Choline Kinase Beta, with a N-terminal His-Tag, expressed in E. coli. Molecular Weight: ~22.6kD Applications: Suitable for use as a positive control, immunogen, SDS-PAGE and Western Blot. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Molekulargewicht: 22.6
UniProt: O55229
Reinheit: 95%.
Formulierung: Supplied as a lyophilized powder in PBS, pH 7.4, 0.01% SKL, 5% trehalose. Reconstitute with sterile 10mM PBS, pH 7.4 to 0.1-1mg/ml. Do not vortex.