Leucyl tRNA Synthetase (LARS), Recombinant, Mouse, His-Tag, GST-Tag

Artikelnummer: USB-050506
Artikelname: Leucyl tRNA Synthetase (LARS), Recombinant, Mouse, His-Tag, GST-Tag
Artikelnummer: USB-050506
Hersteller Artikelnummer: 050506
Alternativnummer: USB-050506-10,USB-050506-50,USB-050506-100,USB-050506-200
Hersteller: US Biological
Kategorie: Molekularbiologie
Applikation: WB
Source: Recombinant protein corresponding to Met1~Ala321 from mouse Leucyl tRNA Synthetase, fused to His-Tag & GST-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~66.7kD Applications: Suitable for use as a positive control, immunogen, SDS-PAGE and Western Blot. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Molekulargewicht: 66.7
UniProt: Q8BMJ2
Reinheit: 95%.
Formulierung: Supplied as a lyophilized powder in PBS, pH 7.4, 0.01% SKL, 5% trehalose. Reconstitute with sterile 10mM PBS, pH 7.4 to 0.1-1mg/ml. Do not vortex