Flagellin, Recombinant, Salmonella enterica Serovar Typhimurium, aa1-495, Highly Active
Artikelnummer:
USB-052405
Hersteller Artikelnummer:
052405
Alternativnummer:
USB-052405-50
Hersteller:
US Biological
Kategorie:
Molekularbiologie
Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It activates the innate immune system through the receptor Toll-like Receptor 5 (TLR5) and the intracellular protein NLRC4 (NLR family CARD domain-containing protein 4). Native recombinant flagellin [FliC] corresponding to aa1-495 from Salmonella enterica Serovar Typhimurium strain ATCC14028. Specificity: Binds to human and mouse TLR5 (Toll-like receptor 5). Molecular Weight: ~54kD (SDS-PAGE) Biological Activity: Activation of TLR5 in human epithelial cell assays based on NF-kappaB luciferase fusions, on expression of IL-8 (CXCL8), CCL20, inducible nitric oxide synthase, beta-defensin 2 and matrilysin (MMP-7). Maturation of dendritic cells in in vitro and in vivo assays. Adjuvant activity on foreign antigens delivered by subcutaneous and intranasal routes in mice. TLR5 can be stimulated in vitro by 052405 at a concentration of 5-50ng/ml and in vivo at a starting concentration of 0.1-1ug/mouse. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
