May be an adherent factor for rickettsial adsorption to the host-cell surface and a determinant of virulence of individual rickettsial strain. It is the major outer membrane protein. Full length recombinant protein corresponding to aa23-521 from Orientia tsutsugamushi (Rickettsia tsutsugamushi) 56kD Type-specific Antigen, fused to 6XHis-Tag at C-terminal, expressed in E. coli. Swiss/Uniprot: P37917 Molecular Weight: ~60kD Amino Acid Sequence: IEFDENSLECGPYAKVGIVGGVLSGVESARLDPADSEGKKHLPLIKGMPFGVTLAAGMTITPGVRAEISAMYLMNVKAEVELGKMGSDANTGTTADASAGVIRKHKKLTPPQPNIMPISIADRDIAVDIPNAAGQGNVDVRAAARIAWLKNYAGIDYYVPDSNNPQGRVVNPVLLNIPQGNPNPAGGGGRAAPAAFDILDHAQWRDVVVGITALSNANKPNVSAVKILSDKISQIYADIKPFANVASVQISETPLPDSASVDQIQNKVQELNKVLEDVRESFDGFILNAFAQPVRLNFQIPQVVQGQGQQPQAAATAQEAAAAAAIRALNDGENNGIIQLYKDLYKLQRNVALKKSMKQLGDELGVDQGQEGGCSKDKKQSDTTAEESKKEGKKGKEIEFDLHMAVGQVKLYADLFTIDSFSVYAGIGAGLAYTHGKIDGKDIKAHTGMVGSLALGVAANVADGVYMDVDAGYLYSFSKIEEKYQMNAFVASAGIRYNF Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
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