| High density lipoprotein, aka the ''good cholesterol, functions as the transit for cholesterol AWAY from blood, tissues, and organs of the body TO the liver where cholesterol breakdown occurs, ultimately helping to remove cholesterol from the arteries. Having HDL levels less than 40mg/dL for men and less than 50mg/dL for women is a major risk factor for heart disease. (Mayo Clinic, 2016) HDL makes up 20%-30% of total cholesterol in the body, a combination of HDL, LDL, and triglycerides, which is typically measured through a blood test. (Harvard University, 2015) Total fasting cholesterol (no eating for 12 hours) should be measured once every five years, at the very least, beginning at the age of 20. (American Heart Association, 2014) Recently, HDL has been found to be an important mediator in the reversal of metabolic dysfunction and the regression of cardiovascular disease due to atherosclerosis. (Klancic, Woodward, Hofmann, & Fisher, 2016). HDL infusions are being tested in clinical trials due to HDLs ability to mediate the reversal of cholesterol transport. HDL infusions are isolated, partially delipidated HDL proteins and native apoAI or genetic variants such as apoAI Milano complexed with phospholipids. These agents have the potential to be applied to a vast range of vascular diseases, especially acute coronary syndromes. (Kingwell & Chapman, 2013) Total Cholesterol: >1,500 mg/dL (Enzymatic) Triglyceride: Reported in mg/dL Triglyceride Ratio: <0.55 (Triglycerides/Total Cholesterol) Electrophoresis: One major band observed, corresponds to HDL Molecular Weight: 175,000-350,000 Suitable for use in: 1. Lipid Panels. 2. Infusion Therapy. 3. HDL-regression clinical trials. 4. Cardiac Research. 5. Immunoassays 6. Research of HDL-raising drugs. 7. Cell culture. 8. Clinical chemistry 9. Testing/assay validation. 10. ELISA, Blotting, etc.. 11. Characterization and manufacturing. Optimal dilutions to be determined by the researcher. Storage and Stability: Thaw quickly, in a 37C water bath, removing as soon as thawed Store at 2-8C for up to one week. Dilute with 0.15M sodium chloride. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
