Lactadherin is a widely distributed glycoprotein (~ 50kD), which was originally characterized due to its association with milk fat/lipid globule membranes. Synonymous names are PAS-6/7, bovine-associated mucoprotein, BA-46, P47, and MFG-E8. Structural hallmarks of lactadherin are the presence of two epidermal growth factor (EGF) homology domains (with an RGD peptide motif in the second EGF domain), and two C domains sharing homology with the discoidin family of lectin domains including the phospholipid-binding domains of blood clotting factors V and VIII. Lactadherin shows preferential binding to phosphatidylserine (L-form) in a calcium independent manner. Purified lactadherin functions as an anticoagulant by blocking phosphatidylserine-containing membrane sites for blood coagulation proteins (10). Fluoresence-labeled lactadherin functions as a sensitive probe for exposed phosphatidylserine on nucleated cells and on stimulated platelets (8, 9). Lactadherin will bind to membranes that have phosphatidylserine content below the threshold for annexin V binding. Localization: Milk (1), also secreted by stimulated macrophages (2), vascular smooth muscle (3), endothelial cells(4) Plasma Concentration: Unknown in normal adults, measurable in women with metastatic breast cancer (5), measurable in newborn calves after milk meal (1). Molecular Weight: 52kD and 47kD (glycosylation variants, PAS-6 and PAS-7 respectively) Extinction Coefficient: E1%, 1cm, 280nm = 16.5 Structure: Single chain with two EGF domains and two C domains. The mouse isoform contains a central proline rich region and the human molecule contains a single EGF domain Percent Carbohydrate: 6%, 13% for glycosylation variants Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molekulargewicht:
52000
Reinheit:
95% (SDS-PAGE)
Formulierung:
Supplied as a liquid in 70mM sodium phosphate, pH 7.0. No preservative added.
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