Heat Shock Protein 60, Recombinant, Human, aa2-573, His-Tag (HSP60, HSPD1, Chaperonin 60, Mitochondrial Matrix Protein P1)
Artikelnummer:
USB-059446
Hersteller Artikelnummer:
059446
Alternativnummer:
USB-059446-50
Hersteller:
US Biological
Kategorie:
Molekularbiologie
Heat shock protein 60 (Hsp60), also known as heat shock protein family D member 1 (HspD1), is an ~60kD protein that functions as a molecular chaperone.1 It belongs to the type I subclass of chaperonins and is found in eubacteria, mitochondria, and chloroplasts where its expression is induced by stress. Hsp60 primarily exists as a heptameric ring that it is converted to a tetradecameric double-ring structure in the presence of ATP.2 Within mitochondria, it associates with its co-chaperone, Hsp10, to form a barrel-like structure and refold proteins that have been shuttled to the mitochondria in an ATP-dependent manner.2,3 Hsp60 also has extramitochondrial functions such as the production of proinflammatory cytokines in human leukocytes and activation of innate immune receptors.4,5 Hsp60 expression is increased in the serum and saliva of patients with type 2 diabetes mellitus and mutations in HSPD1 lead to neurodegenerative diseases.5,6 Full length active recombinant protein corresponding to aa2-573 from human HSP60, fused to His-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~63.27kD Activity Assay: Confirmed by ADP detection assay. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
