SH2D2A (SH2 domain-containing protein 2A, also TSAd and VRAP) is a 52kD protein best described as a multifunctional intracellular adaptor molecule. It is expressed in activated CD4+ and CD8+ T cells, endothelial cells, and fibroblasts. SH2D2A is found in both cytoplasm and nucleus, and has multiple binding partners. After TCR activation, SH2D2A interacts with LCK, prolonging its activity. It also binds to SMAD2 and SMAD3, and constitutively interacts with PI3K and PLCg, thus participating in VEGF:KDR signaling. Human SH2D2A is 389 amino acids (aa) in length and contains one SH2 domain (aa 95-186) and a Pro-rich region (aa 187-389). Phosphorylation occurs on Y39, Y216 and Y305, and on S217 and S296. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. AA Sequence: MEFPLAQICPQGSHEAPIPTFSTFQITDMTRRSCQNLGYTAASPQAPEAASSTGNAERAEEVPGEGSLFLQAETRAWFQKTQAHWLLQHGAAPAWFHGFITRREAERLLEPKPQGCYLVRFSESAVTFVLTYRSRTCCRHFLLAQLRDGRHVVLGEDSAHARLQDLLLHYTAHPLSPYGETLTEPLARQTPEPAGLSLRTEESNFGSKSQDPNPQYSPIIKQGQAPVPMQKEGAGEKEPSQLLRPKPPIPAKPQLPPEVYTIPVPRHRPAPRPKPSNPIYNEPDEPIAFYAMGRGSPGEAPSNIYVEVEDEGLPATLGHPVLRKSWSRPVPGGQNTGGSQLHSENSVIGQGPPLPHQPPPAWRHTLPHNLSRQVLQDRGQAWLPLGPPQ Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
