p53 (Tumor Suppressor Protein, Oncogene Protein) (HRP), IgG2a, Clone: [BP53-12], Mouse, Monoclonal
Artikelnummer:
USB-138582-AF-HRP
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| Artikelname: | p53 (Tumor Suppressor Protein, Oncogene Protein) (HRP), IgG2a, Clone: [BP53-12], Mouse, Monoclonal |
| Artikelnummer: | USB-138582-AF-HRP |
| Hersteller Artikelnummer: | 138582-AF-HRP |
| Alternativnummer: | USB-138582-AF-HRP-100 |
| Hersteller: | US Biological |
| Wirt: | Mouse |
| Kategorie: | Antikörper |
| Applikation: | ELISA, IHC, IP, WB |
| Immunogen: | Full length recombinant protein corresponding to human p53. |
| Recognizes a 53kD protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53 under denaturing and non-denaturing conditions. Its epitope maps within the N-terminus (aa20-25) of p53 oncoprotein. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, re plication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml Immunoprecipitation: 1-2ug/500ug protein lysate Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min RT Optimal dilutions to be determined by the researcher. Positive Control: MDA-MB-231 Cells. Breast or Colon carcinoma Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody. |
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