Recombinant protein corresponding to human IL-6, expressed in E.coli
Interleukin-6 (IL6) is a cytokine that functions in inflammation and the maturation of B cells. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this IL6 is implicated in a wide variety of inflammation-associated disease states, including susceptibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Sandwich ELISA: 1-2ug/ml detects at least 1000pg/ml of recombinant human IL-6 when used with biotinylated antigen affinity purified anti-human IL-6 as the detection antibody at a concentration of approximately 0.1-0.2ug/ml. Western Blot: 0.5-1ug/ml detects human IL-6. Used in conjunction with compatible secondary reagents the detection limit for recombinant human IL-6 is 1-2ng/lane, under reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Klon-Bezeichnung:
[14G17]
Reinheit:
Purified by Protein A affinity chromatography from tissue culture supernatant
Formulierung:
Supplied as a lyophilized powder from PBS. Reconstitute with 500ul sterile ddH2O.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
