Sandwich ELISA quantitative detection kit for Human Adiponectin in cell culture supernates, serum and plasma (heparin, EDTA). Adiponectin (ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin. The standard product used in this kit is the product of gene recombination, consisting of 226 (19-244) amino acids with the molecular mass of 36kD after glycosylation. Assay Range: 1.56ng/ml-100ng/ml Sensitivity: < 60 pg/ml Crossreactivity: There is no detectable cross-reactivity with other relevant proteins. Uniprot ID: A8K660 Entrez Gene ID: 9370 Kit Components: 96-well plate pre-coated with anti-Adiponectin, Human Lyophilized recombinant Adiponectin, Human standard, 10ng/tube, 2Ea USB Cat No Kit Component 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution) 143697 Sample diluent buffer, 30ml 143698 Antibody diluent buffer, 12ml 143699 ABC diluent buffer, 12ml 143700 TMB color developing agent, 10ml 143701 TMB stop solution, 10ml Storage and Handling: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles.
