Sandwich ELISA quantitative detection kit for Human ANG in cell culture supernates, serum and plasma (heparin, EDTA). Angiogenin (Ang) also known as ribonuclease 5 is a protein that in humans is encoded by the ANG gene.[1] Angiogenin is a potent stimulator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease.[2] Hooper et al. (2003) reviewed the evidence that angiogenins are involved in host defense and noted that inflammation provokes upregulated ANG mRNA expression in liver and an increase in detectable ANG protein in serum. Weremowicz et al. (1989, 1990) assigned the human angiogenin gene to chromosome 14q11. Assay Range: 78pg/ml-5000pg/ml Sensitivity: < 12 pg/ml Crossreactivity: There is no detectable cross-reactivity with other relevant proteins. Uniprot ID: P03950 Entrez Gene ID: 283 Kit Components: 96-well plate pre-coated with anti-ANG, Human Lyophilized recombinant ANG, Human standard, 10ng/tube, 2Ea USB Cat No Kit Component 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution) 143697 Sample diluent buffer, 30ml 143698 Antibody diluent buffer, 12ml 143699 ABC diluent buffer, 12ml 143700 TMB color developing agent, 10ml 143701 TMB stop solution, 10ml Storage and Handling: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles.
