Sandwich ELISA quantitative detection kit for Mouse MIP-2 in cell culture supernates, serum and plasma (heparin, EDTA). MIP is a member of the aquaporin family of membrane-bound water channels.1 MIP family proteins are thought to contain 6 TM domains. Sequence analysis suggests that the proteins may have arisen through tandem, intragenic duplication from an ancestral protein that contained 3 TM domains.2 Major intrinsic protein (MIP, also called MP26) is the predominant fiber cell membrane protein of the ocular lens.3 The major intrinsic protein (MIP) of the vertebrate eye lens is the first identified member of a sequence-related family of cell-membrane proteins that appears to have evolved by gene duplication. Several members of the MIP family transport water (aquaporins), glycerol and other small molecules in microbial, plant and animal cells.4 The standard used in this kit is recombinant mouse MIP-2(A28-N100), consisting of 73 amino acids with the molecular mass of 8kD. Assay Range: 15.6pg/ml-1000pg/ml Sensitivity: < 5 pg/ml Crossreactivity: There is no detectable cross-reactivity with other relevant proteins. Uniprot ID: P10889 Entrez Gene ID: 20310 Kit Components: 96-well plate pre-coated with anti-MIP-2, Mouse Lyophilized recombinant MIP-2, Mouse standard, 10ng/tube, 2Ea USB Cat No Kit Component 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution) 143697 Sample diluent buffer, 30ml 143698 Antibody diluent buffer, 12ml 143699 ABC diluent buffer, 12ml 143700 TMB color developing agent, 10ml 143701 TMB stop solution, 10ml Storage and Handling: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles.
