Sandwich ELISA quantitative detection kit for Human MMP13 in cell culture supernates, serum and plasma (heparin). Collagenase 3 is an enzyme that in humans is encoded by the MMP13 gene.[1][2]Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. [3] The expression of MMP13 in osteoarthritic cartilage and its activity against type II collagen indicates that the enzyme plays a significant role in cartilage collagen degradation and must, therefore, form part of a complex target for proposed therapeutic interventions based on collagenase inhibition. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Assay Range: 78pg/ml-5000pg/ml Sensitivity: < 5 pg/ml Crossreactivity: There is no detectable cross-reactivity with other relevant proteins. Uniprot ID: A8K846 Entrez Gene ID: 4322 Kit Components: 96-well plate pre-coated with anti-MMP13, Human Lyophilized recombinant MMP13, Human standard, 10ng/tube, 2Ea USB Cat No Kit Component 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution) 143697 Sample diluent buffer, 30ml 143698 Antibody diluent buffer, 12ml 143699 ABC diluent buffer, 12ml 143700 TMB color developing agent, 10ml 143701 TMB stop solution, 10ml Storage and Handling: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles.
