Sandwich ELISA quantitative detection kit for Human PAI-1 in cell culture supernates, serum and plasma (heparin, EDTA, citrate). Plasminogen activator inhibitor-1 is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), the activators of plasminogen and hence fibrinolysis (the physiological breakdown of blood clots). It is a serine protease inhibitor (serpin) protein (SERPINE1). PAI-1 is mainly produced by the endothelium (cells lining blood vessels), but is also secreted by other tissue types, such as adipose tissue. The PAI-1 gene is located on the seventh chromosome (7q21.3-q22).1 Congenital deficiency of PAI-1 leads to a hemorrhagic diathesis (a tendency to hemorrhage). PAI-1 is present in increased levels in various disease states (such as a number of forms of cancer), as well as in obesity and the metabolic syndrome. It has been linked to the increased occurrence of thrombosis in patients with these conditions. Additionally, it has been shown to interact with ORM1.2 The standard product used in this kit is recombinant human PAI-1 with the molecular mass of 43kD. Assay Range: 156pg/ml-10,000pg/ml Sensitivity: < 10 pg/ml Crossreactivity: There is no detectable cross-reactivity with other relevant proteins. Uniprot ID: P05121 Entrez Gene ID: 5054 Kit Components: 96-well plate pre-coated with anti-PAI-1, Human Lyophilized recombinant PAI-1, Human standard, 10ng/tube, 2Ea USB Cat No Kit Component 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution) 143697 Sample diluent buffer, 30ml 143698 Antibody diluent buffer, 12ml 143699 ABC diluent buffer, 12ml 143700 TMB color developing agent, 10ml 143701 TMB stop solution, 10ml Storage and Handling: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles.
