SIRT1 is an NAD+dependent class III histone deacetylase. It is a member of the sirtuin family of proteins, homologous to the yeast Sir2 protein. It modulates intracellular activities through transcription regulation and functions in several separate cellular functions. SIRT1 deacetylates and inactivates tumor suppressor protein p53, a process regulated by Hypermethylated in Cancer 1 (HIC1) protein to allow cells to bypass apoptosis and survive DNA damage (1, 2). The level of SIRT1 is significantly elevated in mouse and human prostate cancer (3). SIRT1 promotes vascular relaxation by activating endothelial nitric oxide synthase (4). SIRT1 also protects against microgliadependent amyloidbeta toxicity through inhibiting NFKappa b signaling (5). Source: Recombinant protein corresponding to aa1-747, Met1-Ser747, from human Sirtuin 1/SIRT1, fused to 6-His-tag at C-terminal, expressed in E. coli. Molecular Weight: ~110-113kD Endotoxin: <1EU/1ug (LAL method) Biological Activity: Measured by its ability to remove the acetyl group from a fluorogenic peptide substrate Ac-RGK(Ac)-AMC in a coupled assay. Specific Activity: >15 pmol/min/ug Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
