E6AP (E6-Associated Protein) is a HECT domain ubiquitin E3 ligase that ubiquitinates and mediates the proteasomal destruction of substrate proteins. HECT domain ligases use an active site cysteine to accept charged ubiquitin from ubiquitin-E2 thioester complexes for subsequent transfer to substrate proteins, in this way HECT class ligases are distinct from most RING class ligases in that the latter facilitate transfer of ubiquitin from charged E2s directly to substrate proteins without an E3-ubiquitin thioester intermediate. The viral HPV-E6 protein (present in cells infected with human papillomavirus) forms a complex with E6AP to generate a ligase activity that polyubiquitinates the tumor suppressors p53 and p73 and targets them to the 26S proteasome for degradation. This protein contains a C-terminal 6-His tag. Recombinant protein corresponding to Met1 - Leu875 from human His6-E6AP/UBE3A, CF. Molecular Weight: ~102kD Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -70C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
