Synthetic peptide corresponding to 19aa from near the C-terminus of human APC3.
Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC3, also known as CDC27, a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), is a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. APC3 contains a tetratricopeptide repeat (TPR) region and interacts with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1. Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Mouse Liver Tissue Lysate Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Reinheit:
Purified by immunoaffinity chromatography.
Formulierung:
Supplied as a liquid in PBS, 0.02% sodium azide.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
