G-Protein Alpha 11 (GNa11) BioAssay(TM) ELISA Kit (Human)

Produktübersicht

• Artikelname: G-Protein Alpha 11 (GNa11) BioAssay(TM) ELISA Kit (Human)
• Artikelnummer: USB-152593
• Hersteller Artikelnummer: 152593
• Alternativnummer: USB-152593-96
• Hersteller: US Biological
• Kategorie: Kits/Assays

Produktbeschreibung

The G-Protein Alpha 11 (GNa11) BioAssay(TM) ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GNa11 in human tissue homogenates, cell lysates and other biological fluids. Detection Range: 0.156-10ng/ml Sensitivity: 0.055ng/ml Intra-Assay: CV<10% Inter-Assay: CV<12% Kit Components: *152593A: Microtiter Strips, 1x96 wells *152593B:. Standard, 2x1 vial 152593C: Standard Diluent, 1x20ml *152593D: Detection Reagent A, 1x120ul *152593E: Detection Reagent B, 1x120ul 152593F: Assay Diluent A, 1x12ml 152593G: Assay Diluent B, 1x12ml 152593H: TMB Substrate, 1x9ml *152593J: Positive Control, 1x1vial, 2.12ng/ml (Lyophilized) 152593K: Stop Solution, 1x6ml 152593L: Wash Buffer, 30X Precaution: The Stop Solution (152593K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *152593A, *152593B, *152593D, *152593E and *152593J at -20C. After reconstitution of *152593J, store at 4C and use within 5 days. Store all the other components at 4C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Notes on 152593J - Positive Control: Intended Use: The control vials are from a human cell lysates sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly. Characteristics: The control vials contain human GNa11 in human cell lysates sample (lyophilized powder). Note: The human based serum used in this product are screened for HIV, Hepatitis A, B, C, HTLV and Syphilis. Expected Value: 2.12ng/ml Usage: Dissolve with 150ul standard diluent and let stand for 10 minutes. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and samples. Note: Upon receipt store Positive Control vial at -20C. After reconstitution, store Positive Control vial at 4C and use within 5 days. Assay Procedure Summary: 1. Prepare all reagents, samples and standards. 2. Add 100ul standard or sample to each well. Incubate 1 hour at 37C. 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37C. 6. Aspirate and wash 5 times. 7. Add 90ul TMB Substrate. Incubate 10-20 minutes at 37C. 8. Add 50ul Stop Solution. Read at 450nm immediately.