Source: Recombinant Mouse from E. coli Accession No: Q9CR16 Fragment: Lys9~Asp189 (Accession No: Q9CR16) Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- KP SNSKNPRVFF DVDIGGERVG RIVLELFADI VPKTAENFRA LCTGEKGTGS TTGKPLHFKG CPFHRIIKKF MIQGGDFSNQ NGTGGESIYG EKFEDENFHY KHDREGLLSM ANAGPNTNGS QFFITTVPTP HLDGKHVVFG QVIKGLGVAR TLENVEVNGE KPAKLCVIAE CGELKEGDD Epitope Tag: N-terminal Tags: His-tag and T7-tag Molecular Weight: 23.5kD Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70C. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0.. Aliquot to avoid repeated freezing and thawing. Store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Supplied as lyophilized powder from 20mM Tris, 150mM sodium chloride, pH 8.0, 1mM EDTA, 1mM DTT, 5% trehalose, 0.01% sarcosyl, 0.05% Proclin-300. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0 to a concentration of 0.1-1mg/ml. Do not v
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