Recombinant protein corresponding to aa135-340 with an N-terminal His Tag from mouse DSC1 expressed in E. coli Uniprot/Accession: P55849 Predicted Isoelectric Point: 5.1 Predicted Molecular Weight: 24.6kD Accurate Molecular Weight: 24.kD as determined by SDS-PAGE reducing conditions Subcellular Location: Membrane Amino Acid Sequence: RWAPIP CSLMENSLGP FPQHIQQIQS DAAQNYTIFY SISGPGVDKE PYNLFYIEKD TGDIYCTRSI DREQYDQFLV YGYATTADGY APDYPLPLLF KVEDDNDNAP YFETKLTVFS VPENCRSGTS VGQVTAIDKD EPGTLHTRLK YKILQQIPDQ PKHFSIHPDT GVITTTTPLL DREKCDTYKL VMEVRDMGGQ PFGLFTTGTI Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70C. Reconstitute with sterile ddH2O . Aliquot to avoid repeated freezing and thawing. Store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Supplied as lyophilized powder from PBS, pH 7.4, 0.01% sarcosyl, 5% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-0.5mg/ml. Do not vortex.
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