Glutathione S-Transferase Alpha 5 (GSTa5) Recombinant, Rat
Artikelnummer:
USB-154811
Hersteller Artikelnummer:
154811
Alternativnummer:
USB-154811-10,USB-154811-50,USB-154811-200
Hersteller:
US Biological
Kategorie:
Molekularbiologie
Recombinant protein corresponding to aa1-221 with an N-Terminal His Tag frpm rat GSTa5, expressed in E. coli. Uniprot/Accession: P46418. Amino Acid Sequence: MPGKPVLHYF DGRGRMEPIR WLLAAAGVEF EENFLKTRDD LARLRSDGSL MFEQVPMVEI DGMKLVQTKA ILNYIATKYN LYGKDMKERA LIDMYAEGVA DLELMVLYYP YMPPGEKEAS LAKIKDKARN RYFPAYEKVL KSHGQDYLVG NKLSRADVSL VELLYHVEEM DPGIVDNFPL LKALRTRVSN LPTVKKFLQP GSQRKPFDDE KCVESAKKIF S Predicted Isoelectric Point: 8.4 Predicted Molecular Weight: 26.6kD Accurate Molecular Weight: 26kD as determined by SDS-PAGE reducing conditions Subcellular Location: Secreted Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Supplied as lyophilized powder from PBS, pH 7.4, 0.01% sarcosyl, 5% trehalose. Reconstitute with sterile PBS to a concentration of 0.1-1mg/ml. Do not vortex.
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