Sphingosine 1 Phosphate Lyase 1 (SGPL1) Recombinant, Human
Artikelnummer:
USB-156866
Hersteller Artikelnummer:
156866
Alternativnummer:
USB-156866-10,USB-156866-50,USB-156866-200
Hersteller:
US Biological
Kategorie:
Molekularbiologie
Source: Recombinant Human from E. coli Accession No: O95470 Fragment: Gln239~Arg493 (Accession No: O95470) Sequence: MGHHHHHHSG SEF-QS AHAAFNKAAS YFGMKIVRVP LTKMMEVDVR AMRRAISRNT AMLVCSTPQF PHGVIDPVPE VAKLAVKYKI PLHVDACLGG FLIVFMEKAG YPLEHPFDFR VKGVTSISAD THKYGYAPKG SSLVLYSDKK YRNYQFFVDT DWQGGIYASP TIAGSRPGGI SAACWAALMH FGENGYVEAT KQIIKTARFL KSELENIKGI FVFGNPQLSV IALGSRDFDI YRLSNLMTAK GWNLNQLQFP PSIHFCITLL HAR Epitope Tag: N-terminal His-Tag Molecular Weight: 29.9kD Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70C. Reconstitute with sterile sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Supplied as lyophilized powder from 100mM sodium bicarbonate, 500mM sodium chloride, pH 8.3, 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, 0.05% Proclin 300. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml. Do not vortex.
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