Source: Recombinant protein corresponding to Human GMP, Pro143-Ser288, with an N-terminal Ala, expressed in E. coli. Molecular Weight: ~16.5kD Biological Activity: Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. The ED50 for this effect is 0.1-0.6ng/ml. Specific Activity: ~800 IU/ug, which is calibrated against human FGF basic WHO International Standard (NIBSC code: 90/712). Endotoxin: <0.10EU/1ug Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Produced using non-animal reagents in an animal-free laboratory.
