Nectins are a small family of Ca2+-independent immunoglobulin (Ig)-like cell adhesion molecules (CAMs) that control cell adhesion, proliferation, and migration.1-3 Mouse Nectin-2 is a 70 to 78kD type I transmembrane glycoprotein that is found on a variety of cell types.4, 5 The extracellular domain of mouse Nectin-2 (amino acids 32-338) shares 72%, 77%, and 95% amino acid sequence identity with the extracellular domain in human, canine, and rat Nectin-2, respectively. Through its interaction with natural killer cell- and T cell-expressed DNAM-1, it also promotes lymphocyte cytotoxicity and cytokine secretion against both tumors and dendritic cells (DCs) expressing 6, 7. Nectin-2. the immune system eliminates DCs that are inefficient at antigen presentation. Nectin-2 is expressed on epithelium, endothelial cells, Sertoli cells, monocytes, dendritic cells, granulosa cells, mast cells, eosinophils, and fibroblasts.07. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
