PD-1 : PD-L1 (Biotinylated) Inhibitor Screening BioAssay(TM) Kit

Produktübersicht

• Artikelname: PD-1 : PD-L1 (Biotinylated) Inhibitor Screening BioAssay(TM) Kit
• Artikelnummer: USB-171948
• Hersteller Artikelnummer: 171948
• Alternativnummer: USB-171948-1
• Hersteller: US Biological
• Kategorie: Kits/Assays

Produktbeschreibung

Cell signaling through the PD-1 receptor upon binding the PD-L1 ligand attenuates immune responses and is exploited by both tumors and viruses. The PD-1 PD-L1 (Biotin) Inhibitor Screening BioAssay(TM) Kit is designed for screening and profiling inhibitors of this signaling. This kit comes in a convenient 96-well format, with biotin-labeled PD-L1, purified PD-1, streptavidin-labeled HRP, and assay buffer for 96 binding reactions. The key to this kit is the high sensitivity of detection of biotin-labeled PD-L1 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, PD-1 is coated on a 96-well plate. Next, PD-L1 is incubated with PD-1 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader. Application: This kit is useful for screening for inhibitors of PD1 binding to PD-L1. Kit Components: *171948A: PD-L1 (Biotin), 1x5ug *171948B: PD-1, 1x10ug 171948C: Streptavidin (HRP), 1x15ul **171948D: PD-1 Assay Buffer 3X, 1x50ml 171948E: Blocking Buffer, 1x50ml 171948F: HRP Chemiluminescent Substrate A (Transparent bottle), 1x6ml 171948G: HRP Chemiluminescent Substrate B (Brown bottle), 1x6ml 171948H: Microplate, 1x96 wells Storage and Stability: Store *171948A and *171948B at -70C. Store **171948D at -20C. Store other components at 4C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.