SBE Reporter BioAssay(TM) Kit (TGFb/SMAD signaling pathway)

Produktübersicht

• Artikelname: SBE Reporter BioAssay(TM) Kit (TGFb/SMAD signaling pathway)
• Artikelnummer: USB-171993
• Hersteller Artikelnummer: 171993
• Alternativnummer: USB-171993-500
• Hersteller: US Biological
• Kategorie: Kits/Assays

Produktbeschreibung

The SBE Reporter BioAssay(TM) kit is designed for monitoring the activity of TGFbeta/SMAD signaling pathway in the cultured cells. The kit contains transfection-ready SBE luciferase reporter vector, which is a TGFbeta pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized SBE responsive element located upstream of a minimal promoter. The SBE reporter is premixed with constitutively expressing Renilla luciferase vector that serves as internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively expressing Renilla luciferase vector as negative control. The noninducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity. Applications: Monitor TGFbeta signaling pathway activity. Screen activators or inhibitors of TGFbeta/ SMAD signaling pathway. Study effects of RNAi or gene overexpression on the activity of TGFbeta pathway. Components: Reporter (Component A), 1x500ul Negative Control Reporter (Component B), 1x500ul Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.