The Fluorogenic SIRT5 BioAssay(TM) Kit is a complete assay system designed to measure SIRT5 activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent SIRT5 activity measurements. In addition, the kit includes purified SIRT5 enzyme and a SIRT inhibitor, Nicotinamide, for use as a positive and negative control, respectively. The Fluorogenic SIRT5 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only three simple steps on a microtiter plate are needed to analyze the SIRT5 activity level. First, the Sirtuin 5 fluorometric substrate (SIRT substrate 5), containing a succinylated lysine side chain, is incubated with purified SIRT5 enzyme. The desuccinylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader. Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications. Components: SIRT5 human recombinant enzyme, 1x50ug Fluorogenic SIRT5 substrate (5mM), 1x50ul Nicotinamide Adenine Dinucleotide (NAD+) (50mM), 1x50ul Nicotinamide (10mM), 1x500ul 2x SIRT Developer (contains 2mM Nicotinamide), 1x6ml SIRT assay buffer, 1x10ml black, low binding NUNC black microtiter plate Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
