SRE Reporter BioAssay(TM) Kit (MAPK/ERK Signaling Pathway)

Produktübersicht

• Artikelname: SRE Reporter BioAssay(TM) Kit (MAPK/ERK Signaling Pathway)
• Artikelnummer: USB-171997
• Hersteller Artikelnummer: 171997
• Alternativnummer: USB-171997-500
• Hersteller: US Biological
• Kategorie: Kits/Assays

Produktbeschreibung

The SRE Reporter BioAssay(TM) Kit is designed for monitoring the activity of the ERK signaling pathway and the transcriptional activity of SRF in cultured cells. The kit contains a transfection-ready SRE luciferase reporter vector, which is an ERK pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized SRE responsive elements located upstream of a minimal promoter. The SRE reporter is premixed with a constitutively-expressing Renilla luciferase vector that serves as an internal control for transfection efficiency. Applications: Monitor MAPK/ERK signaling pathway activity and SRF-mediated activity. Screen for activators or inhibitors of the MAPK/ERK signaling pathway. Study effects of RNAi or gene overexpression on the activity of the MAPK/ERK pathway. Components: Reporter (Component A), 1x500ul Negative Control Reporter (Component B), 1x500ul Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.